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Protein-coated plates were blocked with 5% skim milk at 37C for 2 h

To construct the modified homozygous strain expressing GAP1 as the counter-selectable marker in the BY4743 background, the KanMX fragment was amplified from pFA6A-KanMX6 [32], and HS fragments for pGAP1 were amplified from yeast genomic DNA and these fragments plus pGSH1 (above) assembled into pRS315 to give pRS315- HS-KanMX-pGSH1-HS

4.2 NOs role in mitochondrial dysfunction and oxidative stress The activity of mitochondrial nitric oxide synthase (mtNOS) is a key driver of mitochondrial dysfunction, oxidative stress, and progression in colorectal cancer (CRC)

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