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In addition, O 2 or H 2 O 2 can also be produced at other sites in the mitochondria, such as some mitochondrial enzymes, including glycerol 3-phosphate dehydrogenase and electron transferring flavoprotein-Q oxidoreductase

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Specifically, the pcDNA3.1-GST DAF -ATP6AP1L transfection resulted in an ATP6AP1L gene expression level of 47 times that of the control, and the affinity cell sorting procedure significantly elevated the apparent expression level in the enriched cells by 253 times that of the control