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One is multi-use safety, whereas the other is pure single-use

All volunteers also received standardized lifestyle coaching on diet and exercise

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time