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To detect a difference in cotinine concentration in smokers of 50 ng/ml (with a mean value of 500 ng/ml and a standard deviation of 200 ng/ml), with an alpha of 5% and a beta of 10% (statistical power of 90%), we estimated that a sample size of 337 smokers would be needed

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For Example: If you smoke one pack of cigarettes (20 sticks) a day, thats equivalent to roughly 300400 puffs from a 20mg nicotine vape