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Ethics statement The animal study was reviewed and approved by China Medical University

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Summary Keywords assisted reproductive technology, capacitation, in vitro fertilization, implantation, live birth Citation Gmez-Elas MD, Luque GM, Oscoz-Susino N, Novero AG, Briski O, Ksparas I, Steeman TJ, Stival C, Lavolpe M, Julianelli V, Geller M, Attie M, Vassena R, Krapf D and Buffone MG (2025) Increased reproductive outcomes after optimized sperm preparation