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Modern English references (Ashmarin 2010, Levitskaya 2004, Myasoedov 2017) provide bibliographic entry points to systematically deepen before designing a protocol, to avoid reinventing results already published 30 years ago in a less accessible language

Recent advances in gene therapy for thalassemia

The mechanism formally parallels the -elimination reaction catalyzed by PLP-dependant enzymes which accept cysteine thioethers as substrates and likely play a role in the renal toxicity and carcinogenicity of halogen-alkenes through their urinary metabolites [125]

It may either be omitted or replaced by Na2- EDTA