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Furthermore, we will discuss how they work and how to apply them in real life

Journal of Strength and Conditioning Research, 24 (5), 12151222

2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes