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While the number of particles in the second reconstruction was much lower than the first, the data were consistent with a lower number of particles in a CT-like conformation (~ 17% of particles) relative to the bypass conformation (55% of particles) compared to the percentage of particles previously observed for the Amy-CTR-Gs complex (note, in the repeat experiment 28% of the particles could not be confidently classified, due to poorly resolved in ECD features) (Supplementary Fig
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The absorbance of each sample was measured at 593 nm, and the concentrations were calculated as recommended and are expressed in nmol/ml
305 A recent study revealed that the cell adhesion molecule KIRREL1 contributes to the recruitment of SAV1 to cellcell contact sites and facilitates the activation of downstream kinases in the Hippo pathway
