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In the standard 2D cell cultures, cells reveal a cobblestone shape, whereas in more advanced models with a dynamic flow, cells mimic the physiologic shape of vascular ECs in vivo, acquiring an elongated shape, due to shear stress [75]

Front Mol Neurosci (2017) 10:203

A rapid, direct stream can damage the delicate peptide structure

As a result, stopping treatment does not appear to cause the same degree of suppression that may occur when hormones are supplied directly from an outside source