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Standard insulin syringes measure in 0.01mL increments (1 unit = 0.01mL), which sets a practical lower limit: concentrations below 100mcg/mL require drawing volumes under 0.05mL (5 units) for typical 2050mcg research doses, introducing significant measurement error

The patient knows what light pressure feels like, knows where to stamp and where to skip, and has a sense of how the serum vial empties through the session