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Cell proliferation assay (CCK-8) The medium in a 96-well plate was replaced with 100 L of the complete medium and Cell Counting Kit-8 (CCK-8) reagent mixture (9:1), and the plate was incubated in 5% CO 2 for 24 h at 37C

Furthermore, this review points out the limitations of traditional antioxidant treatments, such as supplementation of direct antioxidants and enhancement of endogenous antioxidant defense network, and highlights the opportunities brought by new technologies, aiming to provide directions for future research in this field (Figure 1)

& Doenhoff, M

The role of coenzyme A in the biotransformation of 2-arylpropionic acids