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Although both dysregulated hepatic glutathione homeostasis and TNF overproduction are critically involved in the development of NAFLD, whether they can be synergistically implicated in hepatocyte death remains to be clearly defined

The percentage of the different compounds was calculated by the area normalization method, without considering response factors

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The 1 mL assay mixture contained 770 L of 50 mM sodium phosphate (pH 7.0), 100 L of 10 mM GSH, 100 L of 2 mM NADPH, 10 L of 1.125 M sodium azide, 10 L 100 U mL 1 glutathione reductase and 10 L homogenate