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Quantification of tubular necrosis was conducted by an experienced nephrologist, blinded to the experimental conditions

Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

Potential Role in Managing Chronic Illnesses Research suggests that glutathione may play a supportive role in managing conditions like diabetes, Parkinsons disease and autoimmune disorders due to its antioxidant and detoxifying properties

Acknowledgements The authors would like to recognize the contribution of the specimen donors as well as the research entities who have made these analyses possible