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The versatility of the resulting method was demonstrated with small samples of whole-cell lysate from human embryonic kidney (HEK) 293T cells, human neuroblastoma SH-SY5Y cells, mouse brain or human cerebrospinal fluid (CSF)

Combining B12 treatment with folic acid is necessary in some cases, as a deficiency in folate can mask the symptoms of B12 deficiency, and vice versa