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FOXO4-DRI or PBS was added and the cells were transferred to a Heat and CO 2 -controlled incubator, attached to a LSM510 confocal microscope (Zeiss)

We stated that, although the statute provides that an applicable hospital may request an exception up to once every 2 years, we believe that providing a high Medicaid facility the opportunity to request an exception once every 2 years (while also limiting its total growth) balances the Congress' intent to prohibit expansion of physician-owned hospitals with the purpose of the exception to the prohibition on expansion of facility capacity (76 FR 74524)