Endura-Chemically Competent Cells (SOLOs) Buccal Swab DNA Isolation and Stabilisation The reads were aligned to

The reads were aligned to transcripts based upon various length classes and read density plotted relative to the percent distance from the 5´ end of the transcripts

Strand displacement amplification*

Unique funnel design prevents buffer spillages

Approximately 50% of the DNA was lost due to precipitation by egg white lysozyme (EW)

Endura-Chemically Competent Cells (SOLOs) Buccal Swab DNA Isolation and Stabilisation The reads were aligned toEndura Competent Cells Clone repetitive sequences and lentiviral libraries Stabilize direct repeats and create lentiviral constructs Generate lentiviral guide RNA libraries Recommended in CRISPR GeCKO library protocols Choose electrocompetent or chemically competent cells Highest efficiency commercially available cells for lentiviral cloning: over 1 107 cfu g (chem) or 1 1010 cfu g (electro) Transformation of Endura Electrocompetent Cells with GeCKO