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Procollagen I, IL-6 and TIMP-1 detection was carried out with a microplate reader (SpectraMax Plus 384 Microplate Reader, Molecular Devices, San Jos, CA, USA), while MMP-1 detection was performed with a spectrofluorometer (Varioskan Flash, Thermo Electron Corporation, Waltham, MA, USA)

Disruptions in microbial diversity or the overgrowth of pathogenic species can compromise gut barrier function, facilitate systemic inflammation, and contribute to disease progression [45,46,47]

Brown recluse bites may cause an area of dead skin that's called an eschar, which typically sloughs off and leaves an ulcer behind