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J Biol Chem 2005

Pep-B also increased the expression of osteo-/odontogenic genes and proteins, alkaline phosphatase activity and nodule mineralization in LPS-stimulated hDPSCs

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The copper coordination chemistry of GHK-Cu enables controlled cellular copper delivery while preventing the formation of reactive oxygen species characteristic of free copper toxicity, with the peptide's binding affinity (log K = 16.1) positioning it perfectly within the physiological copper transport hierarchy to facilitate controlled metal transfer to enzymatic targets including lysyl oxidase (essential for collagen cross-linking) and superoxide dismutase (critical for antioxidant defense)