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The demand for detailed analysis of the pathogenesis and clinical course of chronic diseases and viral diseases like COVID-19, as well as the use of efficacious therapies with minimal or no side effects are paramount

Cell survival and caspase assays WST-1 (Sigma) or Caspase-Glo 3/7 assay (Promega) were used to measure proliferation and apoptosis of liver cancer cells, respectively

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The lentiviral particles for CBS OPT and L2HGDH OPT constructs were generated similarly with the packaging system as those for sgRNA but selected with puromycin at a final concentration of 1.5 g/ml