Endura-Electrocompetent Cells (DUOs) size:24 rxns 4 × 1010 8

4 × 1010 8

Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling

It is difficult to predict which of the four OverExpress strains – C41(DE3)

tolerance of expression-induced toxicity

Endura-Electrocompetent Cells (DUOs) size:24 rxns 4 × 1010 8Endura Competent Cells Clone repetitive sequences and lentiviral libraries Stabilize direct repeats and create lentiviral constructs Generate lentiviral guide RNA libraries Recommended in CRISPR GeCKO library protocols Choose electrocompetent or chemically competent cells Highest efficiency commercially available cells for lentiviral cloning: over 1 107 cfu g (chem) or 1 1010 cfu g (electro) Transformation of Endura Electrocompetent Cells with GeCKO