This work was funded by the National Institutes of Health Directors Pioneer Award Program, the Howard Hughes Medical Institute, and the Wyss Institute for Biologically Inspired Engineering at Harvard University
Recovery advantage Heal injuries faster Competition pressure "Everyone else is doing it" Detection difficulty Peptides clear quickly, testing isn't universal Legal limbo Possession isn't illegal (unlike steroids) Organizations banning peptides: WADA (Olympics) USADA (US Olympic sports) NCAA (college) NFL, NBA, NHL, MLB UFC, PGA, all major leagues If you're a tested athlete: DON'T RISK IT
To test this hypothesis, we treated HCT116 cells with or without 5FU with a range of ETC inhibitors, spanning CICIV (Fig
GSSG concentration is then measured by spectrophotometry with the GSH recycling method, on the basis of conversion of GSSG to GSH by glutathione reductase and NADPH and reaction with 5,5-dithiobis-(2-nitrobenzoic acid)