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Three subsequent injections followed consisting of 1 g/ml oligomycin (complex V inhibitor), 300 nM FCCP (proton gradient uncoupler), and 100 nM rotenone (complex I inhibitor)

Filters of different diameters were also used to generate liposomes of various sizes to assess the effect of liposome size on mTORC1 kinase activity

They are not interchangeable unless specified always double-check the medication label or protocol to ensure youre using the correct type of diluent

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