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Further dilutions of the = 1.15 g/ml Nycodenz solution are then made by dilution with a simple isoosmotic diluent such as 132 mM NaCl, 5.4 mM MgSO4, 0.5 mM DTT, 10 mg/ml BSA, 15 mM HEPES-Tris, pH 7.4 [2], or 128 mM NaCl, 5 mM KCl, 4 mg/ml BSA, 0.3 mM Ca/Na-EDTA, 5 mM Tris-HCl, pH 7.4 [8] or sometimes the cell suspension medium is used [7,11]

This contributes to pain reduction in many injury protocols