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Since it is difficult to have equal number of crosslinking sites on gels of every stiffness, we provided an excess of surface NHS to ensure equivalent ECM protein coating

CTG reagent (50 L) and 50 L of cell medium were incubated and shaken for 10 min under light with an integration time of 3 s

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In contrast, treatment with MAT significantly inhibits the inflammatory responses caused by ferroptosis and lipid peroxidation in the spinal cord, attenuating central nervous system damage in EAE mice (Feng et al., 2024a)