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Cells were labeled with 2 M fluorescent nitric oxide indicator DFA-FM DA and fluorescence was detected by fluorescent microscope with a standard fluorescein bandpass filter (FITC, 500555 nm)

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In most cases, these patients have seen at least half a dozen doctors: gynecologists, gastroenterologists, dermatologists, ENTs (Ear, Nose, and Throat), as well as their primary care doctor and possibly a chiropractor