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Total chemical synthesis and chemotactic activity of human S100A12 (EN-RAGE)

Aliquots (~0.1 mg) of the target compounds were dissolved in methanolic KOH solution (1.2 mol/L, 80% MeOH), incubated at 50 C for 2 h, then adjusted to pH 5.0 and analyzed by means of UPLC-TOF MS in the negative electrospray mode

What it does is enter cells via a nutrient transporter called PepT1, travel to the nucleus, and block NF-B activation directly.2 That mechanism intracellular rather than receptor-mediated is what makes KPV structurally distinctive among peptide therapies