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In the long term, alcohol consumption favours the development of cardiovascular diseases (arrhythmia, hypertension, etc.) and the appearance of cognitive disorders

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After 30 min, a 100 L of acidic stop solution was added into each well and the absorbance at 450 nm was recorded using a using a Biotek Synergy Neo2 HTS Multi-Mode Microplate Reader (Mnnedorf, Switzerland)