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Following the dissociation, TrypLE Express was inactivated by adding 9 mL of ice-cold Advanced DMEM/F12++ containing 10 M Y-27632 and the cells were collected by centrifugation at 400 g for 5 min at 4 C

From November to April, there is more rain, especially in the lower part of the route, but the landscape is greener and there are fewer tourists

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