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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

Reduced calorie diet combined with NNMT inhibition establishes a metabolic memory

Common sites include: Lower abdomen (most common) Upper thigh Upper arm Pinch a fold of skin, insert the needle at a 45-degree angle, and inject slowly over 2-3 seconds

(Shanghai, China)