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Injectable healing protocol: Dose: 2-3mg daily (higher end of range) Frequency: Daily throughout healing period Duration: Until wound resolves plus 1-2 weeks Injection site: Systemic (not into wound) Topical healing protocol: Concentration: 2-4% in wound-appropriate vehicle Application: 2-3 times daily to wound margins (not directly into open wounds) Duration: Throughout healing process Note: Consult healthcare provider for significant wounds GHK-CU pairs exceptionally well with BPC-157 and TB-500 for wound healing

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To measure the agonist-induced calcium release, 50 L dye loading buffer made of Hanks Balanced Salt Solution (HBSS) with 5 M Fluo-4 (Sigma), 0.2% pluronicacid, and 1% FBS was added to the GHSR-expressing cells and incubated for 1 h at 37 C

In the g:Profiler analysis, the Benjamini-Hochberg FDR method was employed for multiple testing correction, with a threshold set at 0.0001