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The GST-tag is often used to increase the yield of expression and solubility of the target protein, however, removal of the Fig 1

To further map the motif responsible, we performed an alanine scanning mutagenesis of the C-terminal region (residues 87131) in the LYSET-10xGCN4 construct and analyzed subcellular localization in SKMEL30 cells

The most commonly referenced community protocol is 50 mcg per day for 10 consecutive days, followed by 4 weeks off (see dosing guide)

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