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Avoid crushing tablets unless required for your research protocol

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Samples were then desalted again, and fractionated to 12 fractions using a 57-minute linear gradient from 3% to 32% acetonitrile (ACN) in 10 mM ammonium bicarbonate pH 8.0, at a flow rate of 0.25 mL/min, with an Agilent 1100 HPLC

Without identification and characterization of these metabolites, it is not possible to assess their pharmacological activity, inactivity, or possible toxicities as well as potentially identify which enzymes are responsible for their clearance, which is necessary to predict possible drug-drug interactions