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Similarly, inhibition of flux through the cytosolic urea cycle by N -methyl-d,l-aspartate (MDLA) (competitive inhibitor of rate-limiting cytosolic urea cycle enzyme ASS1) (5) treatment also significantly reduced CR levels (Figure 6, F and G, and Supplemental Figure 5, H and I), indicating that the ribosylation of creatinine was dependent upon active cytosolic urea cycle metabolism

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