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This is why research protocols typically use subcutaneous administration shortly before the intended sleep onset

Limited spectral dispersion: due to the relatively uniform environments experienced by residues within IDPs/IDRs, their NMR spectra often exhibit poor dispersion, hindering the ability to resolve individual signals

doi: 10.1134/S0006297918090043 84 ItohY.SuzukiT.MiyataN

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